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Description
Rat ET-2 (Endothelin 2) ELISA KitThe Rat (ET 2) Endothelin 2 ELISA Kit measures Endothelin 2 in Rat samples. The plate has been pre coated with Rat ET 2 antibody. ET 2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ET 2 Antibody is added and binds to ET 2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ET 2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
The Rat (ET-2) Endothelin 2 ELISA Kit measures Endothelin 2 in Rat samples. The plate has been pre-coated with Rat ET-2 antibody. ET-2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ET-2 Antibody is added and binds to ET-2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ET-2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat ET-2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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