SKU: 21956502921

Human TRUB1 (Probable tRNA pseudouridine synthase 1) ELISA Kit

Sale price$234.00 Regular price$260.00
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Description

Human TRUB1 (Probable tRNA pseudouridine synthase 1) ELISA KitThe Human TRUB1 (Probable tRNA pseudouridine synthase 1) ELISA Kit measures TRUB1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TRUB1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Human TRUB1 (Probable tRNA pseudouridine synthase 1) ELISA Kit measures TRUB1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TRUB1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with TRUB1 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of TRUB1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 21956502921

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